other:Article Title: Endurance exercise attenuates juvenile irradiation-induced skeletal muscle functional decline and mitochondrial stress
Article Snippet: The following antibodies were used: rat anti-laminin-α2 (1:1500, Sigma-Aldrich, L0663), DAPI (1:3000), mouse anti-BA-D5 (MyHC-I, IgG2b, 1:40, Developmental Studies Hybridoma Bank (DSHB)), mouse anti-SC-71 (MyHC-IIA, IgG1, 1:40, DSHB), mouse anti-BF-F3 (MyHC-IIB, IgM, 1:40, DSHB), rabbit anti-PGC1α (1:1000, Novus Biologicals), rabbit ant-MCU (1:1000, Cell Signaling, D2Z3B), mouse anti-PMCA (1:2000, Thermo Fisher, 5F10), mouse anti-NCX (1:500, Swant, R3F1), mouse anti-MFN1 (1:500, Neuromab, 75-162), mouse anti-GAPDH (1:50,000, Thermo Fisher, AM4300), mouse anti-CASQ1 (1:5000, Affinity BioReagents, MA3-913), rabbit anti-pan SERCA (1:10,000, Santa Cruz, sc-30110), rabbit anti-VDAC (1:5000, Sigma Aldrich), rabbit anti-DNP (1:150, Sigma Aldrich), mouse anti-3-NT (1:1000, Sigma Aldrich, N5538), Alexa Fluor 405-conjugated goat anti-mouse IgG2b (1:1500, Thermo Fisher, A-21141), Alexa Fluor 488-conjugated goat anti-mouse IgM (1:1500, Thermo Fisher, A-21042), Alexa Fluor 594-conjugated goat anti-mouse IgG1 (1:1500, Thermo Fisher, A-21125), AlexaFluor 488-conjugated goat anti-mouse IgG (1:1500, Thermo Fisher, A-11001), AlexaFluor 488-conjugated goat anti-rabbit IgG (1:1500, Thermo Fisher, A-11034), AlexaFluor 647-conjugated goat anti-rat IgG (1:1500, Thermo Fisher, A-21247), goat anti-rabbit IRDye800 (1:10000, LiCor), goat anti-mouse IRDye800 (1:10,000, LiCor), and goat anti-mouse IRDye700 (1:10,000, LiCor).
Article Title: Voluntary wheel running mitigates disease in an Orai1 gain-of-function mouse model of tubular aggregate myopathy
Article Snippet: The following antibodies were used: rat anti-laminin-α2 (1:1500, Sigma-Aldrich, L0663), mouse anti-BA-D5 (MyHC-I, IgG2b, 1:40, Developmental Studies Hybridoma Bank (DSHB)), mouse anti-SC-71 (MyHC-IIA, IgG1, 1:40, DSHB), mouse anti-BF-F3 (MyHC-IIB, IgM, 1:40, DSHB), mouse anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:50000, Thermo Fisher, AM4300), mouse anti-CASQ1 (1:5000, Affinity BioReagents, MA3-913), rabbit anti-pan SERCA (1:10000, Santa Cruz, sc-30110), rabbit anti-Voltage-dependent anion channel (VDAC) (1:5000, Thermo Fisher, AB10527), rabbit anti-STIM1 (1:3000, Sigma Aldrich, S6197), rat anti-HA (1:3000, Sigma Aldrich, ROAHAHA), rabbit anti-OPA1 (1:1000, Cell Signaling, D6U6N), Alexa Fluor 405-conjugated goat anti-mouse IgG2b (1:1500, Thermo Fisher, A-21141), Alexa Fluor 488-conjugated goat anti-mouse IgM (1:1500, Thermo Fisher, A-21042), Alexa Fluor 594-conjugated goat anti-mouse IgG1 (1:1500, Thermo Fisher, A-21125), AlexaFluor 647-conjugated goat anti-rat IgG (1:1500, Thermo Fisher, A-21247), goat anti-rabbit IRDye800 (1:10000, LiCor), goat anti-mouse IRDye800 (1:10000, LiCor), goat anti-mouse IRDye700 (1:10000, LiCor), and donkey anti-rat IgG-horseradish peroxidase conjugate (1:10,000, Jackson ImmunoResearch).
Article Title: Voluntary Wheel Running Mitigates Disease in an Orai1 Gain-of-Function Mouse Model of Tubular Aggregate Myopathy.
Article Snippet: The following antibodies were used: rat anti-laminin-α2 (1:1500, L0663, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-BA-D5 (MyHC-I, IgG2b, 1:40, Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA, USA), mouse anti-SC-71 (MyHC-IIA, IgG1, 1:40, DSHB, Iowa City, IA, USA), mouse anti-BF-F3 (MyHC-IIB, IgM, 1:40, DSHB, Iowa City, IA, USA), mouse anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:50,000, AM4300, Thermo Fisher, Carlsbad, CA, USA), mouse anti-CASQ1 (1:5000, MA3-913, Affinity BioReagents, Golden, CO, USA), rabbit anti-pan SERCA (1:10,000, sc-30110, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-voltage-dependent anion channel (VDAC) (1:5000, AB10527, Thermo Fisher, Carlsbad, CA, USA), rabbit anti-STIM1 (1:3000, S6197, Sigma Aldrich, St. Louis, MO, USA), rat anti-HA (1:3000, ROAHAHA Sigma-Aldrich, St. Louis, MO, USA,), rabbit anti-OPA1 (1:1000, D6U6N, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-COL1A1 (1:1000, AB765P, EMD Millipore, Burlington, MA, USA), rabbit anti-ACTN2 (1:2000, 14221-1-AP, Proteintech, Rosemont, IL, USA), rabbit anti-CYP2E1 (1:2000, H00001571-D01P, Thermo Fisher, Carlsbad, CA, USA), rabbit antiPPIF (1:2000, 45-5900, Thermo Fisher, Carlsbad, CA, USA), Alexa Fluor 405-conjugated goat anti-mouse IgG2b (1:1500, A-21141, Thermo Fisher, Carlsbad, CA, USA), Alexa Fluor 488-conjugated goat anti-mouse IgM (1:1500, A-21042, Thermo Fisher, Carlsbad, CA, USA), Alexa Fluor 594-conjugated goat anti-mouse IgG1 (1:1500, A-21125, Thermo Fisher, Carls- bad, CA, USA), AlexaFluor 647-conjugated goat anti-rat IgG (1:1500, A-21247, Thermo Fisher, Carlsbad, CA, USA), goat anti-rabbit IRDye800 (1:10,000, LiCor, Lincoln, NE, USA), goat anti-mouse IRDye800 (1:10,000, LiCor, Lincoln, NE, USA), goat anti-mouse IRDye700 (1:10,000, LiCor, Lincoln, NE, USA), and donkey anti-rat IgG-horseradish peroxidase conjugate (1:10,000, Jackson ImmunoResearch, West Grove, PA, USA).
Article Title: Voluntary Wheel Running Mitigates Disease in an Orai1 Gain-of-Function Mouse Model of Tubular Aggregate Myopathy
Article Snippet: The following antibodies were used: rat anti-laminin-α2 (1:1500, L0663, Sigma-Aldrich, St. Louis, MO, USA), mouse anti-BA-D5 (MyHC-I, IgG2b, 1:40, Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA, USA), mouse anti-SC-71 (MyHC-IIA, IgG1, 1:40, DSHB, Iowa City, IA, USA), mouse anti-BF-F3 (MyHC-IIB, IgM, 1:40, DSHB, Iowa City, IA, USA), mouse anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:50,000, AM4300, Thermo Fisher, Carlsbad, CA, USA), mouse anti-CASQ1 (1:5000, MA3-913, Affinity BioReagents, Golden, CO, USA), rabbit anti-pan SERCA (1:10,000, sc-30110, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-voltage-dependent anion channel (VDAC) (1:5000, AB10527, Thermo Fisher, Carlsbad, CA, USA), rabbit anti-STIM1 (1:3000, S6197, Sigma Aldrich, St. Louis, MO, USA), rat anti-HA (1:3000, ROAHAHA Sigma-Aldrich, St. Louis, MO, USA,), rabbit anti-OPA1 (1:1000, D6U6N, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-COL1A1 (1:1000, AB765P, EMD Millipore, Burlington, MA, USA), rabbit anti-ACTN2 (1:2000, 14221-1-AP, Proteintech, Rosemont, IL, USA), rabbit anti-CYP2E1 (1:2000, H00001571-D01P, Thermo Fisher, Carlsbad, CA, USA), rabbit anti-PPIF (1:2000, 45-5900, Thermo Fisher, Carlsbad, CA, USA), Alexa Fluor 405-conjugated goat anti-mouse IgG2b (1:1500, A-21141, Thermo Fisher, Carlsbad, CA, USA), Alexa Fluor 488-conjugated goat anti-mouse IgM (1:1500, A-21042, Thermo Fisher, Carlsbad, CA, USA), Alexa Fluor 594-conjugated goat anti-mouse IgG1 (1:1500, A-21125, Thermo Fisher, Carlsbad, CA, USA), AlexaFluor 647-conjugated goat anti-rat IgG (1:1500, A-21247, Thermo Fisher, Carlsbad, CA, USA), goat anti-rabbit IRDye800 (1:10,000, LiCor, Lincoln, NE, USA), goat anti-mouse IRDye800 (1:10,000, LiCor, Lincoln, NE, USA), goat anti-mouse IRDye700 (1:10,000, LiCor, Lincoln, NE, USA), and donkey anti-rat IgG-horseradish peroxidase conjugate (1:10,000, Jackson ImmunoResearch, West Grove, PA, USA).
Muscles:Article Title: Slow motor neurons resist pathological TDP-43 and mediate motor recovery in the rNLS8 model of amyotrophic lateral sclerosis.
Article Snippet: .. The muscles were cross-sectioned at 10 μm thickness, incubated in 5% FBS in PBS for blocking, and then immunostained using the following primary antibodies: mouse anti-BA-D5 (for myosin heavy chain type I; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-BF-F3 (for myosin heavy chain type IIB; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-SC-71 (for myosin heavy chain type IIA; 2–5 μg/mL, DSHB, U. Iowa) [36]. .. After overnight incubation, tissue sections were washed and then incubated with Alexa Fluor secondary antibodies (1:1000, Molecular Probes) for 2 h. Sections were imaged using a Nikon Eclipse Ni inverted microscope.
Article Title: Slow motor neurons resist pathological TDP-43 and mediate motor recovery in the rNLS8 model of amyotrophic lateral sclerosis
Article Snippet: .. The muscles were cross-sectioned at 10 μm thickness, incubated in 5% FBS in PBS for blocking, and then immunostained using the following primary antibodies: mouse anti-BA-D5 (for myosin heavy chain type I; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-BF-F3 (for myosin heavy chain type IIB; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-SC-71 (for myosin heavy chain type IIA; 2–5 μg/mL, DSHB, U. Iowa) [ ]. .. After overnight incubation, tissue sections were washed and then incubated with Alexa Fluor secondary antibodies (1:1000, Molecular Probes) for 2 h. Sections were imaged using a Nikon Eclipse Ni inverted microscope.
Incubation:Article Title: Slow motor neurons resist pathological TDP-43 and mediate motor recovery in the rNLS8 model of amyotrophic lateral sclerosis.
Article Snippet: .. The muscles were cross-sectioned at 10 μm thickness, incubated in 5% FBS in PBS for blocking, and then immunostained using the following primary antibodies: mouse anti-BA-D5 (for myosin heavy chain type I; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-BF-F3 (for myosin heavy chain type IIB; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-SC-71 (for myosin heavy chain type IIA; 2–5 μg/mL, DSHB, U. Iowa) [36]. .. After overnight incubation, tissue sections were washed and then incubated with Alexa Fluor secondary antibodies (1:1000, Molecular Probes) for 2 h. Sections were imaged using a Nikon Eclipse Ni inverted microscope.
Article Title: Slow motor neurons resist pathological TDP-43 and mediate motor recovery in the rNLS8 model of amyotrophic lateral sclerosis
Article Snippet: .. The muscles were cross-sectioned at 10 μm thickness, incubated in 5% FBS in PBS for blocking, and then immunostained using the following primary antibodies: mouse anti-BA-D5 (for myosin heavy chain type I; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-BF-F3 (for myosin heavy chain type IIB; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-SC-71 (for myosin heavy chain type IIA; 2–5 μg/mL, DSHB, U. Iowa) [ ]. .. After overnight incubation, tissue sections were washed and then incubated with Alexa Fluor secondary antibodies (1:1000, Molecular Probes) for 2 h. Sections were imaged using a Nikon Eclipse Ni inverted microscope.
Blocking Assay:Article Title: Slow motor neurons resist pathological TDP-43 and mediate motor recovery in the rNLS8 model of amyotrophic lateral sclerosis.
Article Snippet: .. The muscles were cross-sectioned at 10 μm thickness, incubated in 5% FBS in PBS for blocking, and then immunostained using the following primary antibodies: mouse anti-BA-D5 (for myosin heavy chain type I; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-BF-F3 (for myosin heavy chain type IIB; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-SC-71 (for myosin heavy chain type IIA; 2–5 μg/mL, DSHB, U. Iowa) [36]. .. After overnight incubation, tissue sections were washed and then incubated with Alexa Fluor secondary antibodies (1:1000, Molecular Probes) for 2 h. Sections were imaged using a Nikon Eclipse Ni inverted microscope.
Article Title: Slow motor neurons resist pathological TDP-43 and mediate motor recovery in the rNLS8 model of amyotrophic lateral sclerosis
Article Snippet: .. The muscles were cross-sectioned at 10 μm thickness, incubated in 5% FBS in PBS for blocking, and then immunostained using the following primary antibodies: mouse anti-BA-D5 (for myosin heavy chain type I; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-BF-F3 (for myosin heavy chain type IIB; 2–5 μg/mL, DSHB, U. Iowa), mouse anti-SC-71 (for myosin heavy chain type IIA; 2–5 μg/mL, DSHB, U. Iowa) [ ]. .. After overnight incubation, tissue sections were washed and then incubated with Alexa Fluor secondary antibodies (1:1000, Molecular Probes) for 2 h. Sections were imaged using a Nikon Eclipse Ni inverted microscope.
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